鲨烯-藿烯环化酶

鲨烯-藿烯环化酶(英語:Squalene-hopene cyclase,缩写SHCEC 5.4.99.17)或称作藿烷-22-醇水裂解酶(hopan-22-ol hydro-lyase,EC 4.2.1.129)属于原核生物中生成五环三萜的环化/变位酶家族,能催化下列两种反应,生成藿烯藿烷-22-醇,两种产物的比例为5比1[1][2][3][4][5]

Squalene-hopene cyclase
鲨烯-藿烯环化酶二聚体的晶体结构
识别码
EC編號 or 4/2/1/129.html 5.4.99.17
CAS号 76600-69-6
数据库
IntEnz IntEnz浏览
BRENDA英语BRENDA BRENDA入口
ExPASy英语ExPASy NiceZyme浏览
KEGG KEGG入口
MetaCyc英语MetaCyc 代谢路径
PRIAM英语PRIAM_enzyme-specific_profiles 概述
PDB RCSB PDB PDBj PDBe PDBsum
鲨烯 22(29)-藿烯
鲨烯 + H2O 藿烷-22-醇


参考文献

  1. ^ Hoshino T, Sato T. Squalene-hopene cyclase: catalytic mechanism and substrate recognition. Chemical Communications. February 2002,   (4): 291–301. PMID 12120044. doi:10.1039/b108995c. 
  2. ^ Hoshino T, Nakano S, Kondo T, Sato T, Miyoshi A. Squalene-hopene cyclase: final deprotonation reaction, conformational analysis for the cyclization of (3R,S)-2,3-oxidosqualene and further evidence for the requirement of an isopropylidene moiety both for initiation of the polycyclization cascade and for the formation of the 5-membered E-ring. Organic & Biomolecular Chemistry. May 2004, 2 (10): 1456–70. PMID 15136801. doi:10.1039/b401172d. 
  3. ^ Sato T, Kouda M, Hoshino T. Site-directed mutagenesis experiments on the putative deprotonation site of squalene-hopene cyclase from Alicyclobacillus acidocaldarius. Bioscience, Biotechnology, and Biochemistry. March 2004, 68 (3): 728–38. PMID 15056909. doi:10.1271/bbb.68.728. 
  4. ^ Reinert DJ, Balliano G, Schulz GE. Conversion of squalene to the pentacarbocyclic hopene. Chemistry & Biology. January 2004, 11 (1): 121–6. PMID 15113001. doi:10.1016/j.chembiol.2003.12.013. 
  5. ^ Pearson A, Budin M, Brocks JJ. Phylogenetic and biochemical evidence for sterol synthesis in the bacterium Gemmata obscuriglobus. Proceedings of the National Academy of Sciences of the United States of America. December 2003, 100 (26): 15352–7. PMC 307571 . PMID 14660793. doi:10.1073/pnas.2536559100. 

外部链接