内输蛋白亚基α-2

位於17號人類染色體的基因

内输蛋白亚基α-2(英语:Importin subunit alpha-2)是人体内的一种蛋白质,为内输蛋白α英语Importin α的一种,由17号染色体上的KPNA2基因编码。[5][6]

内输蛋白亚基α-2
已知的结构
PDB直系同源搜索: PDBe RCSB
识别号
别名KPNA2;, IPOA1, QIP2, RCH1, SRP1alpha, Karyopherin alpha 2, SRP1-alpha, karyopherin subunit alpha 2, PTAC58
外部IDOMIM600685 MGI103561 HomoloGene1708 GeneCardsKPNA2
基因位置(人类
17号染色体
染色体17号染色体[1]
17号染色体
内输蛋白亚基α-2的基因位置
内输蛋白亚基α-2的基因位置
基因座17q24.2起始68,035,636 bp[1]
终止68,047,364 bp[1]
直系同源
物种人类小鼠
Entrez
Ensembl
UniProt
mRNA​序列

NM_002266
​NM_001320611

NM_010655

蛋白序列

NP_001307540
​NP_002257

NP_034785

基因位置​(UCSC)Chr 17: 68.04 – 68.05 MbChr 11: 106.88 – 106.89 Mb
PubMed​查找[3][4]
维基数据
查看/编辑人类查看/编辑小鼠

功能

内输蛋白亚基α-2参与了蛋白质由细胞质转运进细胞核的过程,细胞质中,内输蛋白α可结合具有核定位序列的蛋白质[7],接着再与内输蛋白β结合而形成二聚体,后者可与核孔复合物互动,使二聚体和蛋白质一起进入细胞核中。入核后,内输蛋白二聚体与Ran-GTP结合而将原本结合的蛋白质释出,再回到细胞质中。此过程与Ran循环英语Ran (protein)偶连,并需消耗能量[8]

除了协助蛋白质进入细胞核外,KPNA2可能也参与V(D)J重组英语V(D)J recombination[9]

交互作用

核转运蛋白α-2会与以下的蛋白质产生交互作用

参考文献

  1. ^ 1.0 1.1 1.2 GRCh38: Ensembl release 89: ENSG00000182481 - Ensembl, May 2017
  2. ^ 2.0 2.1 2.2 GRCm38: Ensembl release 89: ENSMUSG00000018362 - Ensembl, May 2017
  3. ^ Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine. 
  4. ^ Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine. 
  5. ^ Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA. Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein. Proceedings of the National Academy of Sciences of the United States of America. Jun 1994, 91 (13): 6156–60. PMC 44157 . PMID 8016130. doi:10.1073/pnas.91.13.6156. 
  6. ^ Weis K, Mattaj IW, Lamond AI. Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences. Science. May 1995, 268 (5213): 1049–53. PMID 7754385. doi:10.1126/science.7754385. 
  7. ^ Moroianu, J; Blobel, G; Radu, A. Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.. Proceedings of the National Academy of Sciences of the United States of America. 1995-03-14, 92 (6): 2008–2011. ISSN 0027-8424. PMC 42412 . PMID 7892216. doi:10.1073/pnas.92.6.2008. 
  8. ^ Goldfarb, David S.; Corbett, Anita H.; Mason, D. Adam; Harreman, Michelle T.; Adam, Stephen A. Importin α: a multipurpose nuclear-transport receptor. Trends in Cell Biology. 2004, 14 (9): 505–514. PMID 15350979. doi:10.1016/j.tcb.2004.07.016. 
  9. ^ Entrez Gene: KPNA2 karyopherin alpha 2 (RAG cohort 1, importin alpha 1). (原始内容存档于2010-12-05). 
  10. ^ Lin CY, Huang PH, Liao WL, Cheng HJ, Huang CF, Kuo JC, Patton WA, Massenburg D, Moss J, Lee FJ. ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli. The Journal of Biological Chemistry. Dec 2000, 275 (48): 37815–23. PMID 10980193. doi:10.1074/jbc.M002470200. 
  11. ^ Perez-Villar JJ, O'Day K, Hewgill DH, Nadler SG, Kanner SB. Nuclear localization of the tyrosine kinase Itk and interaction of its SH3 domain with karyopherin alpha (Rch1alpha). International Immunology. Oct 2001, 13 (10): 1265–74. PMID 11581171. doi:10.1093/intimm/13.10.1265. 
  12. ^ Nagoshi E, Yoneda Y. Dimerization of sterol regulatory element-binding protein 2 via the helix-loop-helix-leucine zipper domain is a prerequisite for its nuclear localization mediated by importin beta. Molecular and Cellular Biology. Apr 2001, 21 (8): 2779–89. PMC 86908 . PMID 11283257. doi:10.1128/MCB.21.8.2779-2789.2001. 
  13. ^ Köhler M, Speck C, Christiansen M, Bischoff FR, Prehn S, Haller H, Görlich D, Hartmann E. Evidence for distinct substrate specificities of importin alpha family members in nuclear protein import. Molecular and Cellular Biology. Nov 1999, 19 (11): 7782–91. PMC 84838 . PMID 10523667. doi:10.1128/mcb.19.11.7782. 
  14. ^ Kutay U, Bischoff FR, Kostka S, Kraft R, Görlich D. Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor. Cell. Sep 1997, 90 (6): 1061–71. PMID 9323134. doi:10.1016/s0092-8674(00)80372-4. 
  15. ^ Braem CV, Kas K, Meyen E, Debiec-Rychter M, Van De Ven WJ, Voz ML. Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal. The Journal of Biological Chemistry. May 2002, 277 (22): 19673–8. PMID 11882654. doi:10.1074/jbc.M112112200. 
  16. ^ Seki T, Tada S, Katada T, Enomoto T. Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL. Biochemical and Biophysical Research Communications. May 1997, 234 (1): 48–53. PMID 9168958. doi:10.1006/bbrc.1997.6535. 
  17. ^ Maiyar AC, Leong ML, Firestone GL. Importin-alpha mediates the regulated nuclear targeting of serum- and glucocorticoid-inducible protein kinase (Sgk) by recognition of a nuclear localization signal in the kinase central domain. Molecular Biology of the Cell. Mar 2003, 14 (3): 1221–39. PMC 151592 . PMID 12631736. doi:10.1091/mbc.E02-03-0170. 

延伸阅读